Ganaxolone

Ganaxolone
Clinical data
ATC code none
Legal status
Legal status
  • investigational
Identifiers
Synonyms CCD-1042
3β-Methyl-3α-ol-5α-pregnan-20-one; 3α-Hydroxy-3β-methyl-5α-pregnan-20-one
CAS Number 38398-32-2 YesY
PubChem (CID) 6918305
ChemSpider 5293511 N
UNII 98WI44OHIQ YesY
KEGG D04300 YesY
ChEMBL CHEMBL161915 N
Chemical and physical data
Formula C22H36O2
Molar mass 332.520 g/mol
3D model (Jmol) Interactive image
 NYesY (what is this?)  (verify)

Ganaxolone is a CNS-selective GABAA modulator that acts on well-characterized targets in the brain known to have anxiolytic and anticonvulsant effects.[1] Ganaxolone protects against seizures in diverse animal models, including the pentylenetetrazol, 6 Hz, and amygdala kindling models.[2][3][4] Ganaxolone is a positive allosteric modulator of the action of the GABAA receptor and, unlike benzodiazepines, there does not appear to be tolerance to the anticonvulsant effects of ganaxolone.[1][5]

Ganaxolone is being investigated for potential medical use in the treatment of epilepsy. It is well tolerated in human trials (with exposure of >1,300 patients), with the commonly reported side effects being somnolence (sleepiness), dizziness, and fatigue.[6]Trials in adults with focal onset seizures and in children with infantile spasms have recently been completed.[7][8][9][10] There are ongoing studies in patients with focal onset seizures, PCDH19 pediatric epilepsy, and behaviors in Fragile X syndrome.[7][8][9][10] There are plans to conduct a study in patients with status epilepticus.

Mechanism of Action

The exact mechanism of action for ganaxolone is unknown; however, results from animal studies suggest that ganaxolone works by blocking seizure propagation and elevating seizure thresholds.[2][3][4]

Ganaxolone is designed to modulate both synaptic and extrasynaptic GABAA receptors to calm over-excited neurons.[1][10] Ganaxolone's activation of the extrasynaptic receptor is an additional mechanism that provides stabilizing effects that potentially differentiates it from other drugs that increase GABA signaling.[1]

Ganaxolone binds to allosteric sites of the GABAA receptor to modulate and open the chloride ion channel, resulting in a hyperpolarization of the neuron.[1] This causes an inhibitory effect on neurotransmission, reducing the chance of a successful action potential (depolarization) from occurring.[1][2][3][4]

Ganaxolone's mechanism is similar to allopregnanolone; however, the addition of the 3β-methyl group to the chemical structure prevents ganaxolone from being metabolized like allopregnanolone into an active hormonal steroid. This makes ganaxolone devoid of undesirable hormonal side effects.[1][10] In binding studies, ganaxolone showed no appreciable affinity for estrogen or progesterone receptors, confirming ganaxolone's lack of hormonal activity.[10]

In animal pharmacokinetic and in vitro studies, ganaxolone is shown to be primarily metabolized by CYP3A.[10]

Clinical trials

Ganaxolone is currently being investigated for potential medical use in the treatment of epilepsy and behavioral disorders.

In Phase I and II clinical trials, ganaxolone has been administered in approximately 1,300 subjects at therapeutically relevant dose levels and treatment regimens for up to two years. [10] In these clinical trials, ganaxolone was generally well tolerated, with no adverse effects on cardiovascular, liver, blood or other systems.[10]

The most common adverse events reported across clinical trials are somnolence (sleepiness), dizziness, and fatigue.[6][10] Most of the adverse events reported in the clinical development program were mild or moderate in severity, dose-related, resolved upon treatment discontinuation, and expected based on ganaxolone pharmacology.[10]

In preclinical and clinical findings, ganaxolone exhibits a relatively safe reproductive toxicity profile, especially in comparison to currently available anti-epileptic drugs.[10]

Focal onset seizures

Ganaxolone has been tested in a randomized placebo-controlled Phase II clinical trial as add on therapy for adults with drug-resistant focal onset seizures. People were randomized to receive either ganaxolone or placebo in addition to their current anti-epileptic drugs. The primary endpoint of the study was the absolute reduction in seizure frequency, with secondary endpoints including the percent change of mean weekly seizure frequency from baseline and responder rate.

In the study, ganaxolone showed a reduction of mean weekly seizure frequency versus the placebo arm during the titration and maintenance period (p=0.042).[10] The data also demonstrated a difference of 20% mean seizure reduction from baseline compared to placebo (p=0.0144).[10] Responder rates were numerically larger in the ganaxolone arm, but did not reach statistical significance in the study (p=0.057).[10] In a post hoc analysis, significantly more ganaxolone treated patients achieved ≥30% improvement in weekly seizure frequency (p=0.027).[10]

Fragile X syndrome

The MIND Institute at the University of California, Davis, in collaboration with Marinus Pharmaceuticals, is conducting an exploratory, randomized, placebo-controlled, Phase II proof-of-concept clinical trial evaluating the effect of ganaxolone on behaviors associated with Fragile X syndrome in approximately 60 children and adolescents.[11][12][13][14]

The MIND Institute has been awarded a medical research grant from the United States Department of Defense to provide funding for this study.[13][14]

See also

References

  1. 1 2 3 4 5 6 7 Carter RB, Wood PL, Wieland S, Hawkinson JE, Belelli D, Lambert JJ, White HS, Wolf HH, Mirsadeghi S, Tahir SH, Bolger MB, Lan NC, Gee KW (Mar 1997). "Characterization of the anticonvulsant properties of ganaxolone (CCD 1042; 3alpha-hydroxy-3beta-methyl-5alpha-pregnan-20-one), a selective, high-affinity, steroid modulator of the gamma-aminobutyric acid(A) receptor". The Journal of Pharmacology and Experimental Therapeutics. 280 (3): 1284–95. PMID 9067315.\
  2. 1 2 3 Kaminski RM, Livingood MR, Rogawski MA (Jul 2004). "Allopregnanolone analogs that positively modulate GABA receptors protect against partial seizures induced by 6-Hz electrical stimulation in mice". Epilepsia. 45 (7): 864–7. doi:10.1111/j.0013-9580.2004.04504.x. PMID 15230714.
  3. 1 2 3 Reddy DS, Rogawski MA (May 2010). "Ganaxolone suppression of behavioral and electrographic seizures in the mouse amygdala kindling model". Epilepsy Research. 89 (2-3): 254–60. doi:10.1016/j.eplepsyres.2010.01.009. PMC 2854307Freely accessible. PMID 20172694.
  4. 1 2 3 Reddy DS, Rogawski MA (May 2010). "Ganaxolone suppression of behavioral and electrographic seizures in the mouse amygdala kindling model". Epilepsy Research. 89 (2-3): 254–60. doi:10.1016/j.eplepsyres.2010.01.009. PMC 2854307Freely accessible. PMID 20172694.
  5. ↑ Reddy DS, Rogawski MA (Dec 2000). "Chronic treatment with the neuroactive steroid ganaxolone in the rat induces anticonvulsant tolerance to diazepam but not to itself". The Journal of Pharmacology and Experimental Therapeutics. 295 (3): 1241–8. PMID 11082461.
  6. 1 2 Monaghan EP, Navalta LA, Shum L, Ashbrook DW, Lee DA (Sep 1997). "Initial human experience with ganaxolone, a neuroactive steroid with antiepileptic activity". Epilepsia. 38 (9): 1026–31. doi:10.1111/j.1528-1157.1997.tb01486.x. PMID 9579942.
  7. 1 2 Nohria V, Giller E (Jan 2007). "Ganaxolone". Neurotherapeutics. 4 (1): 102–5. doi:10.1016/j.nurt.2006.11.003. PMID 17199022.
  8. 1 2 Pieribone VA, Tsai J, Soufflet C, Rey E, Shaw K, Giller E, Dulac O (Oct 2007). "Clinical evaluation of ganaxolone in pediatric and adolescent patients with refractory epilepsy". Epilepsia. 48 (10): 1870–4. doi:10.1111/j.1528-1167.2007.01182.x. PMID 17634060.
  9. 1 2 Bialer M, Johannessen SI, Levy RH, Perucca E, Tomson T, White HS (Jan 2013). "Progress report on new antiepileptic drugs: a summary of the Eleventh Eilat Conference (EILAT XI)". Epilepsy Research. 103 (1): 2–30. doi:10.1016/j.eplepsyres.2012.10.001. PMID 23219031.
  10. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Patroneva A, Kaska E, Tsai J (2015). "Additional responder analyses on a phase 2 study of ganaxolone in patients with partial onset seizure epilepsy". American Epilepsy Society.
  11. ↑ "Fragile X Research and Treatment Center: Clinical Research Studies" (PDF). UC Davis MIND Institute. 10 February 2015. Retrieved 27 January 2016.
  12. ↑ "Ganaxolone Treatment in Children With Fragile X Syndrome". Clinicaltrials.gov. 7 November 2012. Retrieved 27 January 2016.
  13. 1 2 "UC Davis Health System. UC Davis researchers win $3 million grant from U.S. Congress to study fragile X". UC Davis Health System. 8 February 2011. Retrieved 27 January 2016.
  14. 1 2 "UC-Davis Gets $3M DoD Grant to Study Marinus' Ganaxolone in Fragile X Syndrome". Genetic Engineering & Biotechnology News. 13 April 2011. Retrieved 27 January 2016.
This article is issued from Wikipedia - version of the 10/31/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.