Nuclear-inclusion-a endopeptidase
| Nuclear-inclusion-a endopeptidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.4.22.44 | ||||||||
| CAS number | 139946-51-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Nuclear-inclusion-a endopeptidase (EC 3.4.22.44, potyvirus NIa protease) is a protease enzyme found in potyviruses.[1][2][3][4] This enzyme catalyses the following chemical reaction:
- Hydrolyses glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus.
- e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln \ (Ser or Gly) for the enzyme from tobacco etch virus.
The enzyme is used encoded in vectors for the artificial expression of recombinant fusion proteins (see TEV protease).
References
- ↑ Fellers, J.P.; Collins, G.B.; Hunt, A.G. (1998). "The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection". Crop Sci. 38: 1309–1319. doi:10.2135/cropsci1998.0011183x003800050030x.
- ↑ Kim, D.-H.; Choi, K.Y. (1998). "Potyvirus NIa protease". In Barrett, A.J.; Rawlings, N.D.; Woessner, J.F. Handbook of Proteolytic Enzymes. London: Academic Press. pp. 721–723.
- ↑ Takahashi, T.; Nakanishi, M.; Yao, Y.; Uyeda, I.; Serizawa, N. (1998). "Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli". Biosci. Biotechnol. Biochem. 62: 953–958. doi:10.1271/bbb.62.953. PMID 9648226.
- ↑ Kim, D.H.; Hwang, D.C.; Kang, B.H.; Lew, J.; Han, J.S.; Song, B.O.D.; Choi, K.Y. (1996). "Effects of internal cleavages and mutations in the C-terminal region of NIa protease of turnip mosaic potyvirus on the catalytic activity". Virology. 226: 183–190. doi:10.1006/viro.1996.0645. PMID 8955037.
External links
- Nuclear-inclusion-a endopeptidase at the US National Library of Medicine Medical Subject Headings (MeSH)
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