4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
| 4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.2.1.182 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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4-Hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase (EC 3.2.1.182, DIMBOAGlc hydrolase, DIMBOA glucosidase) is an enzyme with systematic name (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside beta-D-glucosidase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
- (1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
- (2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
The enzyme from Triticum aestivum (wheat) has a higher affinity for DIMBOA glucoside than DIBOA glucoside.
References
- ↑ Sue, M.; Ishihara, A.; Iwamura, H. (2000). "Purification and characterization of a hydroxamic acid glucoside β-glucosidase from wheat (Triticum aestivum L.) seedlings". Planta. 210: 432–438. doi:10.1007/s004250050029. PMID 10750901.
- ↑ Sue, M.; Ishihara, A.; Iwamura, H. (2000). "Purification and characterization of a β-glucosidase from rye (Secale cereale L.) seedlings". Plant Sci. 155: 67–74. doi:10.1016/s0168-9452(00)00204-1. PMID 10773341.
- ↑ Czjzek, M.; Cicek, M.; Zamboni, V.; Bevan, D.R.; Henrissat, B.; Esen, A. (2000). "The mechanism of substrate (aglycone) specificity in β-glucosidases is revealed by crystal structures of mutant maize β-glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes". Proceedings of the National Academy of Sciences of the United States of America. 97: 13555–13560. doi:10.1073/pnas.97.25.13555. PMID 11106394.
- ↑ Nikus, J.; Esen, A.; Jonsson, L.M.V. (2003). "Cloning of a plastidic rye (Secale cereale) β-glucosidase cDNA and its expression in Escherichia coli". Physiol. Plant. 118: 337–348. doi:10.1034/j.1399-3054.2003.00118.x.
- ↑ Sue, M.; Yamazaki, K.; Yajima, S.; Nomura, T.; Matsukawa, T.; Iwamura, H.; Miyamoto, T. (2006). "Molecular and structural characterization of hexameric β-D-glucosidases in wheat and rye". Plant Physiol. 141: 1237–1247. doi:10.1104/pp.106.077693. PMID 16751439.
- ↑ Sue, M.; Nakamura, C.; Miyamoto, T.; Yajima, S. (2011). "Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae". Plant Sci. 180: 268–275. doi:10.1016/j.plantsci.2010.09.001. PMID 21421370.
External links
- 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase at the US National Library of Medicine Medical Subject Headings (MeSH)
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