UDP-N-acetylglucosamine 2-epimerase (hydrolysing)
| UDP-N-acetylglucosamine 2-epimerase (hydrolysing) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.2.1.183 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
UDP-N-acetylglucosamine 2-epimerase (hydrolysing) (EC 3.2.1.183, UDP-N-acetylglucosamine 2-epimerase, GNE (gene), siaA (gene), neuC (gene)) is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine hydrolase (2-epimerising).[1][2][3][4] This enzyme catalyses the following chemical reaction
- UDP-N-acetyl-alpha-D-glucosamine + H2O N-acetyl-D-mannosamine + UDP
The enzyme is found in mammalian liver, as well as in some pathogenic bacteria including Neisseria meningitidis and Staphylococcus aureus.
References
- ↑ Stasche, R.; Hinderlich, S.; Weise, C.; Effertz, K.; Lucka, L.; Moormann, P.; Reutter, W. (1997). "A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Molecular cloning and functional expression of UDP-N-acetyl-glucosamine 2-epimerase/N-acetylmannosamine kinase". J. Biol. Chem. 272: 24319–24324. doi:10.1074/jbc.272.39.24319. PMID 9305888.
- ↑ Chou, W.K.; Hinderlich, S.; Reutter, W.; Tanner, M.E. (2003). "Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase". J. Am. Chem. Soc. 125 (9): 2455–2461. doi:10.1021/ja021309g. PMID 12603133.
- ↑ Blume, A.; Ghaderi, D.; Liebich, V.; Hinderlich, S.; Donner, P.; Reutter, W.; Lucka, L. (2004). "UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, functionally expressed in and purified from Escherichia coli, yeast, and insect cells". Protein Expr. Purif. 35 (2): 387–396. doi:10.1016/j.pep.2004.02.013. PMID 15135418.
- ↑ Murkin, A.S.; Chou, W.K.; Wakarchuk, W.W.; Tanner, M.E. (2004). "Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase". Biochemistry. 43 (44): 14290–14298. doi:10.1021/bi048606d. PMID 15518580.
External links
- UDP-N-acetylglucosamine 2-epimerase (hydrolysing) at the US National Library of Medicine Medical Subject Headings (MeSH)
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