TRNA pseudouridine32 synthase
| TRNA pseudouridine32 synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 5.4.99.28 | ||||||||
| CAS number | 430429-15-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
TRNA pseudouridine32 synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine32 uracil mutase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
- tRNA uridine32 tRNA pseudouridine32
The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine746 in 23S rRNA.
References
- ↑ Hoang, C.; Chen, J.; Vizthum, C.A.; Kandel, J.M.; Hamilton, C.S.; Mueller, E.G.; Ferre-D'Amare, A.R. (2006). "Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure". Mol. Cell. 24: 535–545. doi:10.1016/j.molcel.2006.09.017. PMID 17188032.
- ↑ Spedaliere, C.J.; Hamilton, C.S.; Mueller, E.G. (2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues". Biochemistry. 39: 9459–9465. doi:10.1021/bi001079n. PMID 10924141.
- ↑ Raychaudhuri, S.; Niu, L.; Conrad, J.; Lane, B.G.; Ofengand, J. (1999). "Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA". J. Biol. Chem. 274: 18880–18886. doi:10.1074/jbc.274.27.18880. PMID 10383384.
- ↑ Ramamurthy, V.; Swann, S.L.; Spedaliere, C.J.; Mueller, E.G. (1999). "Role of cysteine residues in pseudouridine synthases of different families". Biochemistry. 38: 13106–13111. doi:10.1021/bi9913911. PMID 10529181.
- ↑ Wrzesinski, J.; Nurse, K.; Bakin, A.; Lane, B.G.; Ofengand, J. (1995). "A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for Ψ746 in 23S RNA is also specific for Ψ32 in tRNAPhe". RNA. 1: 437–448. PMID 7493321.
- ↑ Behm-Ansmant, I.; Grosjean, H.; Massenet, S.; Motorin, Y.; Branlant, C. (2004). "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae". J. Biol. Chem. 279: 52998–53006. doi:10.1074/jbc.m409581200. PMID 15466869.
External links
- TRNA pseudouridine32 synthase at the US National Library of Medicine Medical Subject Headings (MeSH)
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