Rubredoxin A

Ruberedoxin A (RubA) is a protein conserved across all studied oxygenic photoautotrophs.[1]

Structure

As of March 2015 there was no crystal structure of RubA although a structure of the homologous protein from a cryptomonad was determined using NMR.[2] Investigation of the gene however indicates that it differs from other known rubredoxins in being bound to the thylakoid membrane via a C-terminal transmembrane helix.[1][3][4]

Function

An investigation of Guillardia theta noted that RubA had a similar distribution to Photosystem II (PSII) and immunological experiments indicated the presence of RubA in PSII complexes isolated from Spinacia oleracea.[3] In Synechocystis sp. PCC 6803 it has been demonstrated that the insertion of an antibiotic cassette into the rubA gene results in a marked decrease in the amount of PSII present,[1] while the same mutation within Chlamydomonas reinhardtii and in Arabidopsis thaliana results in a total absence of PSII.[1]

Conversely, another study performed in Synechococcus sp. PCC 7002 indicated that mutation of the rubA gene interfered with iron-sulphur cluster assembly in Photosystem I (PSI), rather than affecting PSII.[4][5]

References

  1. 1 2 3 4 Calderon, R. H., García-Cerdán, J. G., Malnoë, A., Cook, R., Russell, J. J., Gaw, C., Dent, R. M., de Vitry, C. and Niyogi, K. K. (July 2013). "A Conserved Rubredoxin Is Necessary for Photosystem II Accumulation in Diverse Oxygenic Photoautotrophs". The Journal of Biological Chemistry. 288: 26688–26696. doi:10.1074/jbc.M113.487629.
  2. ↑ Schweimer, K., Hoffmann, S., Wastl, J., Maier, U.G., Rösch, P., and Sticht H. "Solution structure of a zinc substituted eukaryotic rubredoxin from the cryptomonad alga "Guillardia theta"". Protein Science. 9: 1474–1486. doi:10.1110/ps.9.8.1474.
  3. 1 2 Wastl, J., Duin, E. C., Iuzzolino, L., Dörner, W., Link. T., Dau, H., Lingelbach, K. and Maier U. G. "Eukaryotically Encoded and Chloroplast-located Rubredoxin Is Associated with Photosystem II". The Journal of Biological Chemistry. 275: 30058–30068. doi:10.1074/jbc.M004629200.
  4. 1 2 Shen, G., Zhao, J., Reimer, S. K., Antonkine, M. L., Cai, Q., Weiland, S. M., Golbeck, J. H. and Bryant, D. A. "Assembly of Photosystem II: I. Inactivation of The rubA Gene Encoding A Membrane-Associated Rubredoxin In The Cyanobacterium Synechococcus sp. PCC 7002 Causes A Loss of Photosystem I Activity". The Journal of Biological Chemistry. 277: 20343–20354. doi:10.1074/jbc.M201103200.
  5. ↑ Shen, G., Antonkine, M. L., van der Est, A., Vassiliev, I. R., Brettel, K., Bittl, R., Zech, S. G., Zhao, J., Stehlik, D., Bryant, D. A. and Golbeck, J. H. "Assembly of Photosystem I: II. Rubredoxin Is Required for the Assembly of Fx in Synechococcus sp. PCC 7002 As Shown by Optical and EPR Spectroscopy". The Journal of Biological Chemistry. 277: 20355–20366. doi:10.1074/jbc.M201104200.
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