Rhamnogalacturonan hydrolase
| Rhamnogalacturonan hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.2.1.171 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Rhamnogalacturonan hydrolase (EC 3.2.1.171, rhamnogalacturonase A, RGase A, RG-hydrolase) is an enzyme with systematic name rhamnogalacturonan alpha-D-GalA-(1->2)-alpha-L-Rha hydrolase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.
The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
References
- ↑ Petersen, T.N.; Kauppinen, S.; Larsen, S. (1997). "The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix". Structure. 5: 533–544. doi:10.1016/s0969-2126(97)00209-8. PMID 9115442.
- ↑ Kofod, L.V.; Kauppinen, S.; Christgau, S.; Andersen, L.N.; Heldt-Hansen, H.P.; Dorreich, K.; Dalboge, H. (1994). "Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus". J. Biol. Chem. 269: 29182–29189. PMID 7961884.
- ↑ Azadi, P.; O'Neill, M.A.; Bergmann, C.; Darvill, A.G.; Albersheim, P. (1995). "The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase". Glycobiology. 5: 783–789. doi:10.1093/glycob/5.8.783. PMID 8720076.
- ↑ Petersen, T.N.; Christgau, S.; Kofod, L.V.; Kauppinen, S.; Johnson, A.H.; Larsen, S. (1997). "Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus". Acta Crystallogr. D. 53: 105–107. doi:10.1107/s0907444996010785. PMID 15299976.
- ↑ Pitson, S.M.; Mutter, M.; van den Broek, L.A.; Voragen, A.G.; Beldman, G. (1998). "Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus". Biochem. Biophys. Res. Commun. 242: 552–559. doi:10.1006/bbrc.1997.8009. PMID 9464254.
External links
- Rhamnogalacturonan hydrolase at the US National Library of Medicine Medical Subject Headings (MeSH)
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