Poly(3-hydroxyoctanoate) depolymerase

In enzymology, a poly(3-hydroxyoctanoate) depolymerase (EC 3.1.1.76) is an enzyme that catalyzes the chemical reaction that breaks down the polyester poly{oxycarbonyl[(R)-2-pentylethylene]} into smaller oligomers.

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is poly{oxycarbonyl[(R)-2-pentylethylene]} hydrolase. Other names in common use include PHO depolymerase, poly(3HO) depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly(HA) depolymerase, poly(HAMCL) depolymerase, and poly[(R)-3-hydroxyoctanoate] hydrolase.

References

Jendrossek D (2001). "Microbial degradation of polyesters". Adv. Biochem. Eng. Biotechnol. 71: 293–325. doi:10.1007/3-540-40021-4_10. PMID 11217416.
Prieto MA, Garcia JL, Martinez M, Luengo JM (1999). "Novel biodegradable aromatic plastics from a bacterial source Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon". J. Biol. Chem. 274 (41): 29228–41. doi:10.1074/jbc.274.41.29228. PMID 10506180.
Schirmer A, Jendrossek D, Schlegel HG (1993). "Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13". Appl. Environ. Microbiol. 59 (4): 1220–7. PMC 202264. PMID 8476295.

Hydrolase: esterases (EC 3.1)

3.1.1: Carboxylic
ester hydrolases

Lipase

Phospholipase
- A1
- A2
- B

3.1.2: Thioesterase

3.1.3: Phosphatase

3.1.4: Phosphodiesterase

3.1.6: Sulfatase

Nuclease (includes
deoxyribonuclease and
ribonuclease)

3.1.11-16: Exonuclease

Exodeoxyribonuclease	RecBCD

Exoribonuclease	Oligonucleotidase

3.1.21-31: Endonuclease

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease

Endoribonuclease	RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex

either deoxy- or ribo-	Aspergillus nuclease S1 Micrococcal nuclease

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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