Poly(3-hydroxybutyrate) depolymerase
| poly(3-hydroxybutyrate) depolymerase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.1.1.75 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
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In enzymology, a poly(3-hydroxybutyrate) depolymerase (EC 3.1.1.75) is an enzyme that catalyzes the chemical reaction
- poly[(R)-3-hydroxybutanoate]n + H2O poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x; x 1-5
Thus, the two substrates of this enzyme are [[poly[(R)-3-hydroxybutanoate]n]] and H2O, whereas its two products are [[poly[(R)-3-hydroxybutanoate]n-x]] and [[poly[(R)-3-hydroxybutanoate]x; x]].
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is poly[(R)-3-hydroxybutanoate] hydrolase. Other names in common use include PHB depolymerase, poly(3HB) depolymerase, poly[(R)-hydroxyalkanoic acid] depolymerase, poly(HA) depolymerase, poly(HASCL) depolymerase, and poly[(R)-3-hydroxybutyrate] hydrolase.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2D80 and 2D81.
References
- Jendrossek D (2001). "Microbial degradation of polyesters". Adv. Biochem. Eng. Biotechnol. 71: 293–325. doi:10.1007/3-540-40021-4_10. PMID 11217416.
- Prieto MA, Garcia JL, Martinez M, Luengo JM (1999). "Novel biodegradable aromatic plastics from a bacterial source Genetic and biochemical studies on a route of the phenylacetyl-coa catabolon". J. Biol. Chem. 274 (41): 29228–41. doi:10.1074/jbc.274.41.29228. PMID 10506180.