Pentafluorophenyl esters

Pentafluorophenyl (PFP) esters are chemical compounds widely used in attaching fluorophores such as fluorescein[1] or haptens[2] to primary amines in biomolecules. They also are particularly valuable in laboratory peptide synthesis. Pentafluorophenyl esters produce amide bonds as effectively as succinimidyl esters and various similar agents do, but PFP esters are particularly useful because they are less susceptible to spontaneous hydrolysis during conjugation reactions.[3]

References

  1. ↑ Hanai, T.; Hatano, H. (1996). Advances in Liquid Chromatography: 35 Years of Column Liquid Chromatography. World Scientific Publication Co. ISBN 978-981-02-1906-2.
  2. ↑ Deck, M. B.; Sjölin, P.; Unanue, E. R.; Kihlberg, J. (1999). "MHC-Restricted, Glycopeptide-Specific T Cells Show Specificity for Both Carbohydrate and Peptide Residues" (pdf). The Journal of Immunology. 162 (8): 4740–4744. PMID 10202015.
  3. ↑ Katz, J. (1998-12-15). "Advances in Peptide Coupling" (pdf). Harvard University.
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