Neoabietadiene synthase

Neoabietadiene synthase (EC 4.2.3.132, AgAS, PtTPS-LAS) is an enzyme with systematic name (+)-copaly-diphosphate diphosphate-lyase (cyclizing, neoabietadiene-forming).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

(+)-copalyl diphosphate

\rightleftharpoons

neoabietadiene + diphosphate

This enzyme is isolated from Abies grandis (grand fir).

References

↑ Peters, R.J.; Flory, J.E.; Jetter, R.; Ravn, M.M.; Lee, H.J.; Coates, R.M.; Croteau, R.B. (2000). "A'bietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme". Biochemistry. 39: 15592–15602. doi:10.1021/bi001997l. PMID 11112547.
↑ Zhou, K.; Gao, Y.; Hoy, J.A.; Mann, F.M.; Honzatko, R.B.; Peters, R.J. (2012). "Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis". J. Biol. Chem. 287 (9): 6840–6850. doi:10.1074/jbc.M111.337592. PMID 22219188.
↑ Ro, D.K.; Bohlmann, J. (2006). "Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1)". Phytochemistry. 67 (15): 1572–1578. doi:10.1016/j.phytochem.2006.01.011. PMID 16497345.

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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