Monoterpene epsilon-lactone hydrolase

Monoterpene epsilon-lactone hydrolase (EC 3.1.1.83, MLH) is an enzyme with systematic name isoprop(en)ylmethyloxepan-2-one lactonohydrolase.^[1] This enzyme catalyses the following chemical reaction

(1) isoprop(en)ylmethyloxepan-2-one + H₂O

\rightleftharpoons

6-hydroxyisoprop(en)ylmethylhexanoate (general reaction)

(2) 4-isopropenyl-7-methyloxepan-2-one + H₂O

\rightleftharpoons

6-hydroxy-3-isopropenylheptanoate

(3) 7-isopropyl-4-methyloxepan-2-one + H₂O

\rightleftharpoons

6-hydroxy-3,7-dimethyloctanoate

The enzyme catalyses the ring opening of epsilon-lactones in Gram-positive bacterium Rhodococcus erythropolis DCL14.

References

↑ van der Vlugt-Bergmans , C.J.; van der Werf , M.J. (2001). "Genetic and biochemical characterization of a novel monoterpene ε-lactone hydrolase from Rhodococcus erythropolis DCL14". Appl. Environ. Microbiol. 67: 733–741. doi:10.1128/aem.67.2.733-741.2001. PMID 11157238.

3.1.1: Carboxylic
ester hydrolases

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease

Endoribonuclease	RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex

either deoxy- or ribo-	Aspergillus nuclease S1 Micrococcal nuclease

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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