J558L cells

J558L (Mouse B Myeloma) Cell Line

J558L cells are mouse B myeloma cells derived from BALB/c strain isolated by M. Bruggeman and M.S. Neuberger in University of Cambridge.[1][2] It is a stable B cell line used to express antibodies. They are a suspension cell line and are best cultured in Eagle's minimal essential medium with 10% Fetal bovine serum, 0.01 units/ml of penicillin and 0.1 mg/ml streptomycin incubated in 37 °C + 5% CO2 + 90% relative humidity with a cell density of 5x105 cell per 15 ml.

Uses

This cell line is usually used to express antibodies since they can result in antibody titers up to 1 mg under optimum conditions.

References

  1. ↑ Oi, VT; Morrison, SL; Herzenberg, LA; Berg, P (1983). "Immunoglobulin gene expression in transformed lymphoid cells". Proc. Natl. Acad. Sci. U.S.A. 80: 825–9. doi:10.1073/pnas.80.3.825. PMC 393473Freely accessible. PMID 6402777.
  2. ↑ http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=88032902&collection=ecacc_gc

External links

This article is issued from Wikipedia - version of the 5/23/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.