Inorganic phosphate transporter family

The inorganic phosphate transporter (PiT) family is a group of carrier proteins derived from Gram-negative and Gram-positive bacteria, archaea, and eukaryotes.

Function

Functionally-characterized members of the family appear to catalyze inorganic phosphate (Pi) or inorganic sulfate uptake either by H+ or Na+ symport. Both PitA (TC# 2.A.20.1.1) and PitB (TC# 2.A.20.1.2) of E. coli probably catalyze metal ion·phosphate:H+ symport, where Mg2+, Ca2+ or Zn2+ (and probably other divalent cations) can complex with Pi. The mammalian proteins (i.e., TC# 2.A.20.2.7) have been reported to function as viral receptors, but they undoubtedly function as transport proteins as well. For numerous gammaretroviruses, such as the gibbon ape leukemia virus, woolly monkey virus, feline leukemia virus subgroup B, feline leukemia virus subgroup T, and 10A1 murine leukemia virus, this receptor is the human type III sodium-dependent inorganic phosphate transporter, SLC20A1, also known as PiT1.[1]

The malaria parasite, Plasmodium falciparum, grows within its host erythrocyte and induces an increase in the permeability of the erythrocyte membrane to a range of solutes including Na+ and K+. This results in a progressive increase in the concentration of Na+ in the erythrocyte cytosol. The parasite cytosol has a relatively low Na+ concentration, generating a large inward Na+ gradient across the parasite plasma membrane. Saliba et al. (2006) showed that the parasite exploits the Na+ electrochemical gradient to energize the uptake of inorganic phosphate (Pi) with a stoichiometry of 2Na+:1Pi and with an apparent preference for the monovalent over the divalent form of Pi (see TC #2.A.20.2.5).

The generalized transport reactions possibly catalyzed by members of the PiT family are:

  1. HPO42- (out) + [nH+ or Na+] (out) → HPO42- (in) + [nH+ or Na+] (in)
  2. Me2+ · HPO42- (out) + nH+ (out) → Me2+ · HPO42- (in) + nH+ (in)
  3. SO42- (out) + nH+ (out) → SO42- (in) + nH+ (in).

Structure

The molecular sizes of Pit family members are reported to vary from 354 to 681 residues (10-12 TMSs) with the mammalian and Plasmodium proteins exhibiting the largest sizes. The sulfate permease of B. subtilis, CysP, is of 354 residues with 11 putative TMSs.[2] As of early 2016, it appears no crystal structures are available for PiT proteins.

Phylogeny

Phylogenetic grouping of the phosphate transport proteins generally correlates with organismal phylogeny. Thus the fungal, plant, animal and archaeal proteins each cluster separately.[3] However, the tree exhibits two clusters of bacterial phosphate transport proteins. One bacterial cluster is distant from the eukaryotic proteins while the other cluster is close to the plant proteins. Both clusters include proteins from Gram-negative and Gram-positive bacteria. The sulfate permease, CysP (TC# 2.A.20.4.1), is distantly related to the phosphate permeases.

Members of the PiT family arose by a tandem internal gene duplication event. Surprisingly, TopPred predicts a 12 TMS topology for the yeast Pho89 protein, but the homologous regions are not predicted to show similar topological features.[4][5]

See also

References

  1. ↑ Farrell, Karen B.; Tusnady, Gabor E.; Eiden, Maribeth V. (2009-10-23). "New structural arrangement of the extracellular regions of the phosphate transporter SLC20A1, the receptor for gibbon ape leukemia virus". The Journal of Biological Chemistry. 284 (43): 29979–29987. doi:10.1074/jbc.M109.022566. ISSN 1083-351X. PMC 2785626Freely accessible. PMID 19717569.
  2. ↑ Mansilla, M. C.; de Mendoza, D. (2000-04-01). "The Bacillus subtilis cysP gene encodes a novel sulphate permease related to the inorganic phosphate transporter (Pit) family". Microbiology (Reading, England). 146 ( Pt 4): 815–821. doi:10.1099/00221287-146-4-815. ISSN 1350-0872. PMID 10784039.
  3. ↑ Saier, M. H.; Eng, B. H.; Fard, S.; Garg, J.; Haggerty, D. A.; Hutchinson, W. J.; Jack, D. L.; Lai, E. C.; Liu, H. J. (1999-02-25). "Phylogenetic characterization of novel transport protein families revealed by genome analyses". Biochimica et Biophysica Acta. 1422 (1): 1–56. ISSN 0006-3002. PMID 10082980.
  4. ↑ Persson, B. L.; Berhe, A.; Fristedt, U.; Martinez, P.; Pattison, J.; Petersson, J.; Weinander, R. (1998-06-10). "Phosphate permeases of Saccharomyces cerevisiae". Biochimica et Biophysica Acta. 1365 (1-2): 23–30. doi:10.1016/s0005-2728(98)00037-1. ISSN 0006-3002. PMID 9693717.
  5. ↑ Persson, B. L.; Petersson, J.; Fristedt, U.; Weinander, R.; Berhe, A.; Pattison, J. (1999-11-16). "Phosphate permeases of Saccharomyces cerevisiae: structure, function and regulation". Biochimica et Biophysica Acta. 1422 (3): 255–272. doi:10.1016/s0304-4157(99)00010-6. ISSN 0006-3002. PMID 10548719.

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