George M. Church

George Church

George Church in 2012
Born George McDonald Church
(1954-08-28) August 28, 1954[1]
MacDill Air Force Base, Florida[1]
Residence Boston, Massachusetts
Citizenship United States
Nationality United States
Fields Chemistry[2]
Institutions
Alma mater
Thesis Genetic Elements within Yeast Mitochondrial and Mouse Immunoglobulin Introns (1984)
Doctoral advisor Walter Gilbert[3]
Known for Father of synthetic biology
Notable awards
Spouse Ting Wu
Website
arep.med.harvard.edu/gmc

George McDonald Church (born August 28, 1954) is an American geneticist, molecular engineer, and chemist. As of 2015, he is Robert Winthrop Professor of Genetics at Harvard Medical School and Professor of Health Sciences and Technology at Harvard and MIT, and was a founding member of the Wyss Institute for Biologically Inspired Engineering at Harvard.[2][4][5]

Education and early life

George McDonald Church was born on August 28, 1954 on MacDill Air Force Base near Tampa, Florida, and grew up in nearby Clearwater;[4][6][7] he attended high school at the preparatory boarding school Phillips Academy, in Andover, Massachusetts, from 1968 to 1972.[8] He then studied at Duke University, completing a bachelor's degree in zoology and chemistry in two years.[4]

In the fall of 1973, Church began research work at Duke University with assistant professor of biochemistry, Sung-Hou Kim, work that continued a year later with Church in a graduate biochemistry program at Duke on an NSF fellowship.[6][9] As Peter Miller reported for the National Geographic series, "The Innovators":

"As a graduate student at Duke… he used x-ray crystallography to study the three-dimensional structure of "transfer" RNA, which decodes DNA and carries instructions to other parts of the cell. It was groundbreaking research, but Church spent so much time in the lab—up to a hundred hours a week—that he neglected his other classes [in the fall of 1975]".[9]

As a result, Church was not compliant with Duke graduate academic policies, and was withdrawn from the degree program in January 1976. He was told that "[We] hope that whatever problems… contributed to your lack of success… at Duke will not keep you from a successful pursuit of a productive career."[9][10] The work gave rise to publications that include a Proceedings report with Church as lead author on an early model for molecular interactions between the minor groove of double-stranded DNA and β-ribbons of proteins[11][12]

Church returned to graduate work at Harvard University in 1977 under Walter Gilbert,[13] and completed a Ph.D. in biochemistry and molecular biology working on mobile genetic elements within introns of yeast mitochondrial and mouse Immunoglobulin genes (1984).[3]

Career

After completing his doctoral work, Church spent 6 months of 1984 at Biogen, the industrial laboratory site where Prof. Gilbert had relocated a sizable part of his former Harvard group;[4] this was followed soon after by a Life Sciences Research Foundation postdoctoral fellowship at the University of California, San Francisco with Gail R. Martin,[14][15] a member of the National Academy of Sciences and joint-discover of a technique to extract mouse embryonic stem cells.[16][17]

Church joined the Harvard Medical School faculty as an assistant professor in 1986.[4] In his subsequent research career, Church is now the Robert Winthrop Professor of Genetics at Harvard Medical School,[18] and to Member of the Harvard-MIT Health Sciences and Technology faculty, described as a "unique collaboration... integrat[ing] science, medicine and engineering to solve problems in human health" at the Institute for Medical Engineering and Science (IMES) at MIT.[19] As well, he was a founding member of the Wyss Institute for Biologically Inspired Engineering at Harvard University.[4] In these positions Church, as of May 2014, is reported to have co-authored more than 330 publications, 60 patents, and a popular science book.[20]

Church has also served as director of the Center on Bioenergy Technology at Harvard, funded by a multiyear award from the U.S. Department of Energy. and of the Center of Excellence in Genomic Science (CEGS) at Harvard, funded by a P50-type award from the National Human Genome Research Institute (NHGRI), a part of the National Institutes of Health.[21]

Research

Church is known for his professional contributions in the sequencing of genomes and interpreting such data, in synthetic biology and genome engineering, and in an emerging area of neuroscience that proposes to map brain activity and establish a "functional connectome." Among these, Church is known for pioneering the specialized fields of personal genomics and synthetic biology. He has co-founded commercial concerns spanning these areas, and others from green and natural products chemistry to infectious agent testing and fuel production, including Knome, LS9, and Joule Unlimited (respectively, human genomics, green chemistry, and solar fuel companies). As of 2015, according to Google Scholar,[2] his most cited research has been published in peer reviewed scientific journals including PNAS,[22][23][24][25] Nature genetics,[26][27][28] nature reviews genetics [29] the Intelligent Systems for Molecular Biology (ISMB) conference,[30] Nature Biotechnology,[31][32][33][34] Science,[35][36][37][38][39][40] the Journal of Molecular Biology,[41][42][43] the Pacific Symposium on Biocomputing (PSB) conference,[44] the Journal of Bacteriology,[45]Nature,[46] Nature Methods,[47] Genome Biology,[48] Bioinformatics,[49][50] PLOS Genetics,[51] and Nucleic Acids Research.[52]

Genome sequencing and interpretation technologies

With Walter Gilbert, Church published the first direct genomic sequencing method in 1984.[53][54] Described in that publication were the cyclic application of fluids to a solid phase alternating with imaging, plus avoidance of bacterial cloning, strategies that are still used in current dominant Next-Generation Sequencing technologies. These technologies began to affect genome-scale sequencing 2005.[55] Church also helped initiate the Human Genome Project in 1984.[56] He invented the broadly applied concepts of molecular multiplexing and barcode tags.[57] Technology transfer from his Harvard laboratory of automated sequencing and software to Genome Therapeutics Corp. resulted in the first bacterial genome sequence and first commercial genome (the human pathogen Helicobacter pylori) in 1994.[58] Church was also co-inventor of nanopore sequencing in 1995, which are now commercially available (e.g. Oxford Nanopore Technologies), but not in the form embodied in Church's contribution to the original patents.[59]

To aid in the interpretation and sharing of genomes, Church, in 2005, initiated the Personal Genome Project (PGP),[60] which provides the world’s only open-access human genome and trait data sets.[61][62][63] Eight trios (mother, father, and child) from the Personal Genome Project are in the process of being chosen to act as the primary genome standards (Reference Materials) for the NIST+FDA genomeinabottle.org program.[64]

Synthetic biology and genome engineering

He has co-developed "genome engineering" technologies since 1997 via either general homologous recombination (recA and lambda-red) [65] or via sequence-specific nucleases.[66] Since 2004, his team has developed use of DNA array (aka DNA chip) synthesizers for combinatorial libraries and assembling large genome segments.[67] He co-developed Multiplex Automated Genome Engineering (MAGE) and optimized CRISPR/Cas9 discovered by Jennifer Doudna and Emanuelle Charpentier for engineering a variety of genomes ranging from yeast to human.[66] His laboratory's use of CRISPR in human induced pluripotent stem cells (hiPS) is the latest contender for precise gene therapy.[68]

His team is the first to tackle a genome-scale change in the genetic code.[69] This was done in a 4.7 million basepair genome of an industrially useful microbe (E. coli) with the goal of making a safer and more productive strain; this strain uses non-proteinogenic amino acids in proteins and is metabolically and genetically isolated from other species.

He has co-invented several uses for DNA, including detectors for dark matter -- Weakly interacting massive particles (WIMPs),[70] anti-cancer "nano-robots",[71] and strategies for digital data storage that are over a million times denser than conventional disk drives.[72] Together with polymerase, DNA can be used to sense and store variation in photons, nucleotides, or ions.[73]

The BRAIN initiative

He was part of a team of six[73] who, in a 2012 scientific commentary, proposed a Brain Activity Map, later named BRAIN Initiative (Brain Research through Advancing Innovative Neurotechnologies).[74] They outlined specific experimental techniques that might be used to achieve what they termed a "functional connectome", as well as new technologies that will have to be developed in the course of the project,[73] including wireless, minimally invasive methods to detect and manipulate neuronal activity, either utilizing microelectronics or synthetic biology. In one such proposed method, enzymatically produced DNA would serve as a "ticker tape record" of neuronal activity.[73][75]

Woolly Mammoth cloning

In March 2015, Church and his genetics research team at Harvard successfully copied woolly mammoth genes into the genome of an Asian elephant. Using the CRISPR DNA editing technique, his group spliced genetic segments from frozen mammoth specimens, including genes from the ears, subcutaneous fat, and hair attributes, into the DNA of skin cells from a modern elephant. This marked the first time that woolly mammoth genes had been functionally active since the species became extinct.[76] Their work has not been subject to peer review, however. Church stated that "Just making a DNA change isn't that meaningful. We want to read out the phenotypes." To do that, the team plans to perform further tests to get the hybrid cells into becoming specialized tissues, and from there attempting to turn the hybrid elephant/mammoth skin cells into hybrid embryos that can be grown in artificial wombs.

Technology transfer and translational impact

Church has co-founded 9 companies, including Veritas Genetics (human genomics, 2014, with Mirza Cifric, Preston Estep, Yining Zhao, Joe Thakuria), Warp Drive Bio (natural products, 2011, with Greg Verdine and James Wells), Alacris (cancer systems therapeutics, 2010, with Hans Lehrach, Bernhard Herrmann, and Shahid Imran), Knome (human genomics, 2007, with Jorge Conde and Sundar Subramaniam),[77] Pathogenica (microbe and viral NGS diagnostics, 2009, with Yemi Adesokan),[78] AbVitro (immunomes, 2010, with Francois Vigneault and Mirza Cifric), Gen9 Bio (synthetic biology, 2009, with Joseph Jacobson and Drew Endy), EnEvolv (Genome Engineering), Joule Unlimited (SolarFuels, 2007, with Noubar Afeyan and David Berry), and LS9 (green chemistry, 2005, with Chris Somerville, Jay Keasling, Vinod Khosla, Noubar Afeyan, and David Berry)[79][80][81]

He has participated in technology development, licensing patents and advising most of the Next-Generation Sequencing companies, including Complete Genomics, Life Technologies, Illumina, Danaher Corporation, Roche Diagnostics, Pacific Biosciences, Genia, and Nabsys.[81]

Support of open consent

Church spearheaded the concept and implementation of open access sequencing hardware[82] and shareable human medical data.[63] He has noted the potential for re-identification of human research participants and the tendency for consent forms to be opaque – proposing an alternative "open consent" mechanism.[61][62] He has participated in the Presidential Commission for the Study of Bioethical Issues,[83] cautioning about the risk of synthetic DNA and proposing risk-reduction via licensing and surveillance.[84][85] His laboratory has a major bio-safety engineering focus.[69]

Church at TED 2010, picture by Steve Jurvetson.

Support of open education

He has been an early advocate of online, open education since 2002. He is advisor to the Personal Genetics Education Project[86] and has spent a day teaching at The Jemicy School.[87] He has championed citizen science, especially in the fields of synthetic biology and personal genomics.[62] Since 2008, his team has been hosting an annual Genomes, Environments and Traits (GET) Conference with free online videos.[88]

Controversies

In response to a question from Der Spiegel, Church speculated that it could be technically possible to make a Neanderthal by reconstructing the DNA of a Neanderthal and modifying living human cells accordingly.[89] Other media such as The Daily Mail fabricated a "Want-Ad" and quote for Church as having said: "Now I need an adventurous female human." [90] Church pointed out that he was not working on such a project.[91][92]

Popular science

In his science and popular efforts, Church has also promoted open access genome sequencing and shareable human medical data, as well as online, open education and citizen science. Controversy found Church in early 2013, in response to his spoken speculations as to what was required to engineer the birth of a Neanderthal.

Church authored the 2012 NewScientist "top science book," Regenesis: How Synthetic Biology Will Reinvent Nature and Ourselves with Ed Regis.[93][94] He has participated in news interviews and videos including at TED, TEDx,[95][96][97] and TEDMED venues, at PBS's Charlie Rose,[98] Faces of America, and NOVA, as well as at PopSci, EG, and The Colbert Report.[99] He is a regular contributor to Edge.org publications and videos.[100]

Awards and honors

Church has received accolades including election to the National Academy of Sciences (in 2011),[4][101] and the National Academy of Engineering (in 2012).[102] He received the American Society for Microbiology Promega Biotechnology Research Award and the heptannual Bower Award and Prize for Achievement in Science of the Franklin Institute.[103] He authored the NewScientist "top science book," Regenesis (on synthetic biology) with Ed Regis. Church is a regular contributor to Edge.org and has appeared widely in the media, including TED venues, NOVA, Faces of America, and Charlie Rose on PBS, and on The Colbert Report.

Other honors include the Triennial International Steven Hoogendijk Award in 2010 and the Scientific American Top 50 twice (for “Designing artificial life” in 2005 and "The $1000 genome" in 2006).[104][105] Newsweek picked Church for their 2008 “Power of Ideas” recognition in the category of Medicine (for the Personal Genome Project).[106] In September 2010, Dr Church was honored for his work in genetics with the Mass High Tech All-Star Award.[107]

He is a member of the Research Advisory Board of SENS Research Foundation.[108]

Personal life

Church is married to fellow Harvard Medical School faculty in genetics, Ting Wu.[109]

Church has been outspoken in his support of following a vegan lifestyle, for reasons concerned with health, and with environmental and moral issues. When asked about his dietary choice, Church replied, "I've been vegan off-and-on since 1974 when I was inspired by participating in an MIT nutritional study, and quite strictly since 2004." He goes on to elaborate 4 reasons:

"medical (cholesterol in fish & dairy), energy conservation (up to 20-fold impact), cruelty ("organic" animals are deprived of medicines that humans use), and risks of spreading pathogens (not just the flu)… [noting that] veganism is an issue for which personal and global love of life, health and wealth align. It's a pity to lose parts of our humanity and planet just due to a lack of recipes."[110][dead link]

In the context of the Personal Genome Project, journalists at Forbes and Wired have noted Church's openness about his health issues, including dyslexia, narcolepsy, and high cholesterol (one of the motivations for his vegan diet).[111][112]

Further reading

References

  1. 1 2 "Church, George". Biography Reference Bank. The H. W. Wilson Company. 2010. Retrieved 2011-12-10.
  2. 1 2 3 George M. Church's publications indexed by Google Scholar
  3. 1 2 Church, George (1984). Genetic Elements within Yeast Mitochondrial and Mouse Immunoglobulin Introns (Sequence, Enhancer, Technique) (PhD thesis). Harvard University. OCLC 13285113.
  4. 1 2 3 4 5 6 7 8 Nair, P. (2012). "Profile of George M. Church". Proceedings of the National Academy of Sciences. 109 (30): 11893–11895. Bibcode:2012PNAS..10911893N. doi:10.1073/pnas.1204148109. PMC 3409755Freely accessible. PMID 22474375.
  5. ↑ George M. Church's publications indexed by the Scopus bibliographic database, a service provided by Elsevier. (subscription required)
  6. 1 2 David C. Brock, 2008, "George M. Church," at Chemical Heritage Foundation: Discover, Collections, Oral Histories, at , accessed 26 February 2015.
  7. ↑ David Ewing Duncan, 2010, "On a Mission to Sequence the Genomes of 100,000 People: The geneticist George Church advises or licenses technology to most companies involved in sequencing, The New York Times, June 7, 2010, see , accessed 26 February 2015.
  8. ↑ Alex Salton, 2009, "Geneticist George Church ’72 Sought Independence at PA," The Phillipian, April 17, 2009, see , accessed 2 March 2015.
  9. 1 2 3 Peter Miller, 2015, "News, The Innovators Project: George Church, The Future Without Limits," National Geographic (online), see , accessed 26 February 2015.
  10. ↑ Duke University Graduate School, Office of the Dean, 1976, "Dear Mr. Church…", January 16, 1976, private letter from W.G. Katzenmeyer, Associate Dean, to George McDonald Church, in the archives of G.M. Church, see . accessed 4 March 2015.
  11. ↑ G. M. Church, J. L. Sussman & S.-H. Kim, 1977, "Secondary structural complementarity between DNA and proteins," Proc. natn. Acad. Sci. U.S.A. 74:1458−1462, see , accessed 4 March 2015.
  12. ↑ Commenting on the new Wayne Anderson, Brian Matthews, et al. structure of a Cro repressor-DNA complex, and on the new David McKay and Thomas Steitz structure of a CAP-cAMP complex; David Davies, 1981, "Two DNA-binding proteins," Nature 290:736f, see , accessed 4 March 2015.
  13. ↑ Jeffrey Perkel, 2013, "BioTechniques: Celebrating 30 Years of Methods Development," BioTechniques 55(5), November 2013, 227–230, see , accessed 21 March 2014.
  14. ↑ LSRF, 2015, "Resources, 1983 Fellow George Church," see , accessed 26 February 2015.
  15. ↑ LSRF, 2015, "Fellows:Alumni, George Church (1984)," see , accessed 26 February 2015.
  16. ↑ Elie Dolgin, 2009, "Stem cell rat race," in The Scientist (magazine), April 1, 2009, see , accessed 26 February 2015.
  17. ↑ Martin G (December 1981). "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.". Proc Natl Acad Sci USA. 78 (12): 7634–8. Bibcode:1981PNAS...78.7634M. doi:10.1073/pnas.78.12.7634. PMC 349323Freely accessible. PMID 6950406.
  18. ↑ Heidi Legg, 2014, "Harvard Professor George Church and the future of genomics," at BetaBoston, a Boston Globe site (online), December 25, 2014, see , accessed 2 March 2015.
  19. ↑ HST, 2015, "People, HST Faculty: George M Church," Harvard-MIT Health Sciences and Technology Program, see and , accessed 6 March 2015.
  20. ↑ mit media lab, 2014, "Media Lab Conversations Series: George Church," in "Joi Ito, Neil Gershenfeld, and Joe Jacobson in conversation with George Church," May 2, 2014, see [www.media.mit.edu/events/2014/05/02/media-lab-conversations-series-george-church], accessed 6 March 2014.
  21. ↑ NHGRI, 2015, Active Centers of Excellence in Genomic Science Awards: Causal Transcriptional Consequences of Human Genetic Variation (P50 HG005550, George M. Church, Harvard University), see , accessed 26 February 2015.
  22. ↑ Church, G.; Gilbert, W. (1984). "Genomic sequencing". Proceedings of the National Academy of Sciences of the United States of America. 81 (7): 1991–1995. Bibcode:1984PNAS...81.1991C. doi:10.1073/pnas.81.7.1991. PMC 345422Freely accessible. PMID 6326095.
  23. ↑ Segrè, D.; Vitkup, D.; Church, G. M. (2002). "Analysis of optimality in natural and perturbed metabolic networks". Proceedings of the National Academy of Sciences of the United States of America. 99 (23): 15112–15117. Bibcode:2002PNAS...9915112S. doi:10.1073/pnas.232349399. PMC 137552Freely accessible. PMID 12415116.
  24. ↑ Chae, H. Z.; Robison, K; Poole, L. B.; Church, G; Storz, G; Rhee, S. G. (1994). "Cloning and sequencing of thiol-specific antioxidant from mammalian brain: Alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes". Proceedings of the National Academy of Sciences of the United States of America. 91 (15): 7017–21. Bibcode:1994PNAS...91.7017C. doi:10.1073/pnas.91.15.7017. PMC 44329Freely accessible. PMID 8041738.
  25. ↑ Kim, J.; Krichevsky, A.; Grad, Y.; Hayes, G.; Kosik, K.; Church, G.; Ruvkun, G. (2004). "Identification of many microRNAs that copurify with polyribosomes in mammalian neurons". Proceedings of the National Academy of Sciences of the United States of America. 101 (1): 360–365. Bibcode:2003PNAS..101..360K. doi:10.1073/pnas.2333854100. PMC 314190Freely accessible. PMID 14691248.
  26. ↑ Tavazoie, S; Hughes, J. D.; Campbell, M. J.; Cho, R. J.; Church, G. M. (1999). "Systematic determination of genetic network architecture". Nature Genetics. 22 (3): 281–5. doi:10.1038/10343. PMID 10391217.
  27. ↑ Pilpel, Y; Sudarsanam, P; Church, G. M. (2001). "Identifying regulatory networks by combinatorial analysis of promoter elements". Nature Genetics. 29 (2): 153–9. doi:10.1038/ng724. PMID 11547334.
  28. ↑ Ge, H; Liu, Z; Church, G. M.; Vidal, M (2001). "Correlation between transcriptome and interactome mapping data from Saccharomyces cerevisiae". Nature Genetics. 29 (4): 482–6. doi:10.1038/ng776. PMID 11694880.
  29. ↑ Shendure, J; Mitra, R. D.; Varma, C; Church, G. M. (2004). "Advanced sequencing technologies: Methods and goals". Nature Reviews Genetics. 5 (5): 335–44. doi:10.1038/nrg1325. PMID 15143316.
  30. ↑ Cheng, Y; Church, G. M. (2000). "Biclustering of expression data". Proceedings / ... International Conference on Intelligent Systems for Molecular Biology ; ISMB. International Conference on Intelligent Systems for Molecular Biology. 8: 93–103. PMID 10977070.
  31. ↑ Tompa, M; Li, N; Bailey, T. L.; Church, G. M.; De Moor, B; Eskin, E; Favorov, A. V.; Frith, M. C.; Fu, Y; Kent, W. J.; Makeev, V. J.; Mironov, A. A.; Noble, W. S.; Pavesi, G; Pesole, G; Régnier, M; Simonis, N; Sinha, S; Thijs, G; Van Helden, J; Vandenbogaert, M; Weng, Z; Workman, C; Ye, C; Zhu, Z (2005). "Assessing computational tools for the discovery of transcription factor binding sites". Nature Biotechnology. 23 (1): 137–44. doi:10.1038/nbt1053. PMID 15637633.
  32. ↑ Roth, F. P.; Hughes, J. D.; Estep, P. W.; Church, G. M. (1998). "Finding DNA regulatory motifs within unaligned noncoding sequences clustered by whole-genome mRNA quantitation". Nature Biotechnology. 16 (10): 939–45. doi:10.1038/nbt1098-939. PMID 9788350.
  33. ↑ Ball, M. P.; Li, J. B.; Gao, Y; Lee, J. H.; Leproust, E. M.; Park, I. H.; Xie, B; Daley, G. Q.; Church, G. M. (2009). "Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells". Nature Biotechnology. 27 (4): 361–8. doi:10.1038/nbt.1533. PMC 3566772Freely accessible. PMID 19329998.
  34. ↑ Selinger, D. W.; Cheung, K. J.; Mei, R; Johansson, E. M.; Richmond, C. S.; Blattner, F. R.; Lockhart, D. J.; Church, G. M. (2000). "RNA expression analysis using a 30 base pair resolution Escherichia coli genome array". Nature Biotechnology. 18 (12): 1262–8. doi:10.1038/82367. PMID 11101804.
  35. ↑ Mali, P.; Yang, L.; Esvelt, K. M.; Aach, J.; Guell, M.; Dicarlo, J. E.; Norville, J. E.; Church, G. M. (2013). "RNA-Guided Human Genome Engineering via Cas9". Science. 339 (6121): 823–826. Bibcode:2013Sci...339..823M. doi:10.1126/science.1232033. PMC 3712628Freely accessible. PMID 23287722.
  36. ↑ Shendure, J.; Porreca, J.; Reppas, B.; Lin, X.; McCutcheon, P.; Rosenbaum, M.; Wang, D.; Zhang, K.; Mitra, D.; Church, G. M. (Sep 2005). "Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome". Science. 309 (5741): 1728–1732. Bibcode:2005Sci...309.1728S. doi:10.1126/science.1117389. ISSN 0036-8075. PMID 16081699.
  37. ↑ Ephrussi, A; Church, G. M.; Tonegawa, S; Gilbert, W (1985). "B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo". Science. 227 (4683): 134–40. Bibcode:1985Sci...227..134E. doi:10.1126/science.3917574. PMID 3917574.
  38. ↑ Drmanac, R; Sparks, A. B.; Callow, M. J.; Halpern, A. L.; Burns, N. L.; Kermani, B. G.; Carnevali, P; Nazarenko, I; Nilsen, G. B.; Yeung, G; Dahl, F; Fernandez, A; Staker, B; Pant, K. P.; Baccash, J; Borcherding, A. P.; Brownley, A; Cedeno, R; Chen, L; Chernikoff, D; Cheung, A; Chirita, R; Curson, B; Ebert, J. C.; Hacker, C. R.; Hartlage, R; Hauser, B; Huang, S; Jiang, Y; et al. (2010). "Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays". Science. 327 (5961): 78–81. Bibcode:2010Sci...327...78D. doi:10.1126/science.1181498. PMID 19892942.
  39. ↑ Sommer, M. O. A.; Dantas, G.; Church, G. M. (2009). "Functional Characterization of the Antibiotic Resistance Reservoir in the Human Microflora". Science. 325 (5944): 1128–1131. Bibcode:2009Sci...325.1128S. doi:10.1126/science.1176950. PMID 19713526.
  40. ↑ Friedland, A. E.; Lu, T. K.; Wang, X; Shi, D; Church, G; Collins, J. J. (2009). "Synthetic gene networks that count". Science. 324 (5931): 1199–202. Bibcode:2009Sci...324.1199F. doi:10.1126/science.1172005. PMC 2690711Freely accessible. PMID 19478183.
  41. ↑ Hughes, J. D.; Estep, P. W.; Tavazoie, S; Church, G. M. (2000). "Computational identification of cis-regulatory elements associated with groups of functionally related genes in Saccharomyces cerevisiae". Journal of Molecular Biology. 296 (5): 1205–14. doi:10.1006/jmbi.2000.3519. PMID 10698627.
  42. ↑ Sussman, J. L.; Holbrook, S. R.; Warrant, R. W.; Church, G. M.; Kim, S. H. (1978). "Crystal structure of yeast phenylalanine transfer RNA. I. Crystallographic refinement". Journal of Molecular Biology. 123 (4): 607–30. doi:10.1016/0022-2836(78)90209-7. PMID 357742.
  43. ↑ Robison, K; McGuire, A. M.; Church, G. M. (1998). "A comprehensive library of DNA-binding site matrices for 55 proteins applied to the complete Escherichia coli K-12 genome". Journal of Molecular Biology. 284 (2): 241–54. doi:10.1006/jmbi.1998.2160. PMID 9813115.
  44. ↑ Chen, T; He, H. L.; Church, G. M. (1999). "Modeling gene expression with differential equations". Pacific Symposium on Biocomputing: 29–40. PMID 10380183.
  45. ↑ Link, A. J.; Phillips, D.; Church, G. M. (1997). "Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: Application to open reading frame characterization". Journal of Bacteriology. 179 (20): 6228–6237. PMC 179534Freely accessible. PMID 9335267.
  46. ↑ Wang, H. H.; Isaacs, F. J.; Carr, P. A.; Sun, Z. Z.; Xu, G; Forest, C. R.; Church, G. M. (2009). "Programming cells by multiplex genome engineering and accelerated evolution". Nature. 460 (7257): 894–8. Bibcode:2009Natur.460..894W. doi:10.1038/nature08187. PMC 4590770Freely accessible. PMID 19633652.
  47. ↑ Porreca, G. J.; Zhang, K.; Li, J. B.; Xie, B.; Austin, D.; Vassallo, S. L.; Leproust, E. M.; Peck, B. J.; Emig, C. J.; Dahl, F.; Gao, Y.; Church, G. M.; Shendure, J. (2007). "Multiplex amplification of large sets of human exons". Nature Methods. 4 (11): 931–6. doi:10.1038/nmeth1110. PMID 17934468.
  48. ↑ Choe, S. E.; Boutros, M; Michelson, A. M.; Church, G. M.; Halfon, M. S. (2005). "Preferred analysis methods for Affymetrix Gene Chips revealed by a wholly defined control dataset". Genome Biology. 6 (2): R16. doi:10.1186/gb-2005-6-2-r16. PMC 551536Freely accessible. PMID 15693945.
  49. ↑ Aach, J; Church, G. M. (2001). "Aligning gene expression time series with time warping algorithms". Bioinformatics (Oxford, England). 17 (6): 495–508. doi:10.1093/bioinformatics/17.6.495. PMID 11395426.
  50. ↑ Teichmann, S. A.; Chothia, C; Church, G. M.; Park, J (2000). "Fast assignment of protein structures to sequences using the intermediate sequence library PDB-ISL" (PDF). Bioinformatics (Oxford, England). 16 (2): 117–24. doi:10.1093/bioinformatics/16.2.117. PMID 10842732.
  51. ↑ Kettler, G. C.; Martiny, A. C.; Huang, K; Zucker, J; Coleman, M. L.; Rodrigue, S; Chen, F; Lapidus, A; Ferriera, S; Johnson, J; Steglich, C; Church, G. M.; Richardson, P; Chisholm, S. W. (2007). "Patterns and implications of gene gain and loss in the evolution of Prochlorococcus". PLoS Genetics. 3 (12): e231. doi:10.1371/journal.pgen.0030231. PMC 2151091Freely accessible. PMID 18159947.
  52. ↑ Bulyk, M. L.; Johnson, P. L.; Church, G. M. (2002). "Nucleotides of transcription factor binding sites exert interdependent effects on the binding affinities of transcription factors". Nucleic Acids Research. 30 (5): 1255–61. doi:10.1093/nar/30.5.1255. PMC 101241Freely accessible. PMID 11861919.
  53. ↑ Church GM, Gilbert W (1984). "Genomic sequencing". Proceedings of the National Academy of Sciences of the United States of America. 81 (7): 1991–1995. Bibcode:1984PNAS...81.1991C. doi:10.1073/pnas.81.7.1991. PMC 345422Freely accessible. PMID 6326095.
  54. ↑ Saluz HP, Jiricny J, Jost JP (1986). "Genomic sequencing reveals a positive correlation between the kinetics of strand-specific DNA demethylation of the overlapping estradiol/glucocorticoid-receptor binding sites and the rate of avian vitellogenin mRNA synthesis". Proc. Natl. Acad. Sci. U.S.A. 83 (19): 7167–71. Bibcode:1986PNAS...83.7167S. doi:10.1073/pnas.83.19.7167. PMC 386676Freely accessible. PMID 3463957. Direct genomic sequencing, first described by Church and Gilbert (15) and further developed in our laboratory (16), overcomes the disadvantages inherent to the use of restriction enzymes.
  55. ↑ Shendure J, Porreca GJ, Reppas NB, Lin X, McCutcheon JP, Rosenbaum AM, Wang MD, Zhang K, Mitra RD, Church GM (2005). "Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome". Science. 309 (5741): 1728–32. Bibcode:2005Sci...309.1728S. doi:10.1126/science.1117389. PMID 16081699.
  56. ↑ Cook-Deegan RM (1989). "The Alta summit, December 1984". Genomics. 5 (3): 661–663. doi:10.1016/0888-7543(89)90042-6. PMID 2613249.
  57. ↑ Church GM, Kieffer-Higgins S (1988). "Multiplex DNA sequencing". Science. 240 (4849): 185–188. Bibcode:1988Sci...240..185C. doi:10.1126/science.3353714. PMID 3353714.
  58. ↑ "Capitalizing on the genome". Nature Genetics. 13 (1): 1–5. 1996. doi:10.1038/ng0596-1. PMID 8673083.
  59. ↑ "Genia Technologies Collaborates with Professors Jingyue Ju at Columbia and George Church at Harvard to Develop a Nanopore-based Sequencing Platform". 3 Oct 2012.
  60. ↑ Church GM (2005). "The Personal Genome Project". Molecular Systems Biology. 1 (1): E1–E3. doi:10.1038/msb4100040. PMC 1681452Freely accessible. PMID 16729065.
  61. 1 2 Ian L. Marpuri (8 Apr 2013). "Researchers explore genomic data privacy and risk (NIH NHGRI)".
  62. 1 2 3 Angrist M (Nov 2009). "Eyes wide open: the Personal Genome Project, citizen science and veracity in informed consent". Personalized Medicine. 6 (6): 691–699. doi:10.2217/pme.09.48. PMC 3275804Freely accessible. PMID 22328898.
  63. 1 2 "Personal Genome Project". Retrieved 25 May 2013.
  64. ↑ "Okay, You've Sequenced My Genome: Are You Sure You Got it Right?". NGS Leaders.
  65. ↑ Link AJ, Phillips D, Church GM (1997). "Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: Application to open reading frame characterization". Journal of Bacteriology. 179 (20): 6228–6237. PMC 179534Freely accessible. PMID 9335267.
  66. 1 2 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM (Feb 15, 2013). "RNA-guided human genome engineering via Cas9". Science. 339 (6121): 823–6. Bibcode:2013Sci...339..823M. doi:10.1126/science.1232033. PMC 3712628Freely accessible. PMID 23287722.
  67. ↑ Tian J, Gong H, Sheng N, Zhou X, Gulari E, Gao X, Church G (2004). "Accurate Multiplex Gene Synthesis from Programmable DNA Chips". Nature. 432 (7020): 1050–4. Bibcode:2004Natur.432.1050T. doi:10.1038/nature03151. PMID 15616567.
  68. ↑ Matthew Herper (19 Mar 2013). "This Protein Could Change Biotech Forever". Forbes.
  69. 1 2 Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, Kraal L, Tolonen AC, Gianoulis TA, Goodman DB, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, Church GM (2011). "Precise Manipulation of Chromosomes in vivo Enables Genome-wide Codon Replacement". Science. 333 (6040): 348–53. Bibcode:2011Sci...333..348I. doi:10.1126/science.1205822. PMID 21764749.
  70. ↑ "Revolutionary 'DNA Tracking Chamber' Could Detect Dark Matter". Technology Review. 2 Jul 2012.
  71. ↑ Belle Dumé (17 Feb 2012). "DNA nanorobot delivers drugs". Physics World.
  72. ↑ Robert Lee Hotz (16 Aug 2012). "Future of Data: Encoded in DNA". Wall Street Journal.
  73. 1 2 3 4 Alivisatos AP, Chun M, Church GM, Greenspan RJ, Roukes ML, Yuste R (June 2012). "The Brain Activity Map Project and the Challenge of Functional Connectomics" (PDF). Neuron. 74 (6): 970–974. doi:10.1016/j.neuron.2012.06.006. PMC 3597383Freely accessible. PMID 22726828. Retrieved February 22, 2013.
  74. ↑ John Markoff (17 Feb 2013). "Obama Seeking to Boost Study of Human Brain". NY Times.
  75. ↑ Zamft BM, Marblestone AH, Kording K, Schmidt D, Martin-Alarcon D, Tyo K, Boyden ES, Church G (August 2012). "Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing". Plos One. 7 (8): e43876. Bibcode:2012PLoSO...743876Z. doi:10.1371/journal.pone.0043876. PMC 3425509Freely accessible. PMID 22928047. Retrieved February 27, 2013.
  76. ↑ Sarah Fecht (24 March 2014), Woolly Mammoth DNA Successfully Spliced Into Elephant Cells, Popular Science
  77. ↑ Dickinson, Boonsri (2010-06-10). "Geneticist George Church: Sequencing human genome 'high priority' for China". Smart Planet. Retrieved 2010-08-18.
  78. ↑ Julia Karow (2010-07-06). "Pathogenica Bets on Next-Gen Sequencing for Fast, Multiplexed Pathogen Detection". GenomeWeb. Retrieved 2011-09-06.
  79. ↑ Duncan, David (7 Jun 2010). "Scientist at Work: George M. Church - On a Mission to Sequence the Genomes of 100,000 People". The New York Times. Retrieved 2010-08-18.
  80. ↑ "Biofuel startup raises $5 million". San Francisco Business Times. 12 Mar 2007.
  81. 1 2 "Tech Transfer, Advisory Roles, and Funding Sources".
  82. ↑ "Open, Affordable Sequencing". Retrieved 25 May 2013.
  83. ↑ "Presidential Commission for the Study of Bioethical Issues". 8 Jul 2010.
  84. ↑ "A Synthetic Biohazard Non-proliferation Proposal". Retrieved 25 May 2013.
  85. ↑ Church G (Nov 24, 2005). "Let us go forth and safely multiply". Nature. 438 (7067): 423. Bibcode:2005Natur.438..423C. doi:10.1038/438423a. PMID 16306966.
  86. ↑ "Personal Genetics Education Project". Retrieved 25 May 2013.
  87. ↑ "Jemicy School for Dyslexia, David Malin Lecture". 11 Nov 2010.
  88. ↑ "Genomes, Environments and Traits (GET) Conference". Retrieved 25 May 2013.
  89. ↑ "How DNA will be construction material of the future". Der Spiegel. 18 Jan 2013.
  90. ↑ "Wanted: 'Adventurous woman' to give birth to Neanderthal man". London: Daily Mail. 20 Jan 2013.
  91. ↑ Gary J. Remal (22 Jan 2013). "Harvard professor blasts Neanderthal clone baby rumor on Web". Boston Herald.
  92. ↑ "Spiegel responds to brouhaha over neanderthal clone". Der Spiegel. 23 Jan 2013.
  93. ↑ Church George (2012). Regenesis. New York: Basic Books. ISBN 0-465-02175-1.
  94. ↑ "The top 10 science books of 2012". NewScientist.
  95. ↑ TEDx, 2012, "TEDxCambridge: George Church on genomics and human diversity," March 21, 2012, see , accessed 4 March 2014.
  96. ↑ TEDx, 2013, "Hybridizing with extinct species: George Church at TEDxDeExtinction," May 8, 2013, see , accessed 4 March 2014.
  97. ↑ TEDx, 2013, "DNA [as detectors], George-Church-at-TEDxCERN," May 24, 2013, see , accessed 4 March 2014.
  98. ↑ charlierose.com, 2009, "Personalized Medicine, …GUESTS/AFFILIATIONS: ...Steven Pinker, Harvard University, George Church, Harvard Medical School, Personal Genome Project," air date June 19, 2009, see and , accessed 4 March 2015.
  99. ↑ "Church Lab News". Retrieved 2010-09-08.
  100. ↑ "Church Writings, Master Class and Videos on Edge.org". Retrieved 25 May 2013.
  101. ↑ "National Academy of Sciences Members and Foreign Associates Elected". National Academies. 2011. Retrieved February 17, 2012.
  102. ↑ "National Academy of Engineering Elects 66 Members and 10 Foreign Associates". National Academies. 2012. Retrieved February 17, 2012.
  103. ↑ "Bower Award and Prize for Achievement in Science". Franklin Institute. 2011. Retrieved December 23, 2011.
  104. ↑ "American Society for Microbiology honors George M. Church". 8 Jun 2009.
  105. ↑ "International Steven Hoogendijk Award". 2010.
  106. ↑ "The Power of Ideas". Newsweek. 19 Dec 2008.
  107. ↑ "All-Star Awards". Mass High Tech. 2010-09-08. Retrieved 2010-09-08.
  108. ↑ Zealley, Ben (March 7, 2013). "SRF's Research Advisory Board Welcomes Dr. George Church". Retrieved 18 August 2014.
  109. ↑ "Do inventors get enough respect in science?". The Boston Globe. 2013-02-07.
  110. ↑ "George Church". Archived from the original on January 18, 2013. Retrieved 17 June 2013.
  111. ↑ Herper, Matthew (27 April 2009). "Going To Church". Forbes Magazine.
  112. ↑ Thomas Goetz (2008-07-08). "How the Personal Genome Project Could Unlock the Mysteries of Life". Wired.
This article is issued from Wikipedia - version of the 11/9/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.