Daniel A. Portnoy

Daniel A. Portnoy
Born 1956
Syracuse, New York
Occupation academic, microbiologist

Daniel A. Portnoy (born 1956 in Syracuse, NY) is a microbiologist, the Edward E. Penhoet Distinguished Chair in Global Public Health and Infectious Diseases, and a Professor of Biochemistry, Biophysics and Structural Biology and in the School of Public Health at the University of California, Berkeley.[1] He is one of the world’s foremost experts on Listeria monocytogenes, the bacterium that causes the severe foodborne illness Listeriosis. He has made seminal contributions to multiple aspects of bacterial pathogenesis, cell biology, innate immunity, and cell mediated immunity using L. monocytogenes as a model system and has helped to push forward the use of attenuated L. monocytogenes as an immunotherapeutic tool in the treatment of cancer.

Education and early career

Dr. Portnoy got his start in microbiology in the lab of Dr. Sydney Rittenberg working on Bdellovibrio as an undergraduate at UCLA where he earned a B.A. in Bacteriology in 1978. He next earned his Ph.D. in 1983 under the tutelage of Stanley Falkow first at the University of Washington finishing at Stanford University. In the Falkow Lab, he worked on the conserved virulence plasmids in Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis,[2] and discovered what turned out to be the first effectors of type III secretion.[3] To further his appreciation of host cells, he did his postdoctoral fellowship in the Zanvil Cohn Laboratory of Cellular Physiology and Immunology at the Rockefeller University in New York, working with Jay Unkeless and Jeff Ravetch. At Rockefeller University he worked on macrophage Fc receptors and lysosomal proteases.

Contributions to Listeria monocytogenes biology

Cartoon and electron micrographs from Dr. Portnoy's seminal observation that L. monocytogenes utilizes host actin to spread cell-to-cell. J Cell Biol 2002 Aug 158(3) 409-14, Figure 1.

In 1986 Dr. Portnoy started his independent lab at Washington University in St. Louis where he began his studies on Listeria monocytogenes. In 1988, he joined the Department of Microbiology at the University of Pennsylvania, where he collaborated with Dr. Lewis Tilney in the Biology Department and made the observation that L. monocytogenes spreads from one cell to another by exploiting a host cell system of actin polymerization.[4] His lab also defined the role of the listerial hemolysin in mediating dissolution of phagosomes.[5] In collaboration with Dr. Philip Youngman, he showed that expression of the L. monocytogenes hemolysin by Bacillus subtilis led to its growth inside of host cells.[6] Dr. Portnoy collaborated with Dr. Yvonne Paterson, who also arrived at Penn in 1988, on the use of L. monocytogenes as a recombinant vector-based vaccine for the induction of cell-mediated immunity.[7][8] Both Drs. Paterson and Portnoy went on to work with biotech companies to develop vaccines for both cancer and infectious disease applications.[9][10] Numerous clinical trials based on their discoveries have shown promising results as immunotherapeutic treatments for cancer.

In 1997, Dr Portnoy moved to UC Berkeley where his lab continues to examine fundamental aspects of L. monocytogenes biology, and has expanded to focus on both innate and acquired immunity in the context of Listeria infection. Dr. Portnoy and collaborators have shown that immune cells recognize cyclic di-AMP, a novel and essential bacterial signaling molecule, secreted by L. monocytogenes through multidrug resistance efflux transporters.[11][12] Dr. Portnoy and Dr. Russell Vance identified that STING was the host receptor of cyclic-di-nucleotides (CDNs) that leads to the production of type I interferon and other co-regulated genes.[13] Modified CDNs are now being evaluated for clinical application as adjuvants and for cancer immunotherapy.

Honors and awards

Dr. Portnoy has been awarded a number of honors over the course of his career including the Eli Lilly and Company Research Award in Microbiology & Immunology,[14] NIH Merit Award, Fellow of the American Academy of Microbiology, Senior Scholar Award in Global Infectious Diseases from the Ellison Foundation, and numerous honorary keynote lectures. In 2013, Portnoy’s contributions were recognized by his election to the National Academy of Sciences[15]

Personal life

Dr. Portnoy has three children, Eleanor Sophie Portnoy (born 1996), Herschel Michael Portnoy (born 1999), and Anna Rose Portnoy (born 2001). His father, Bernard Portnoy, M.D. (1929-2015) was a Professor of pediatrics and infectious diseases at USC; his mother, Roslyn Portnoy (born 1931) lives in Dana Point, CA and sister, Deborah Brown (born 1953) lives in Sherman Oaks, CA.

References

  1. ↑ "Directory Detail | Department of Molecular & Cell Biology". mcb.berkeley.edu. Retrieved 2016-09-01.
  2. ↑ Portnoy, D A; Falkow, S (1981-12-01). "Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis.". Journal of Bacteriology. 148 (3): 877–883. ISSN 0021-9193. PMC 216287Freely accessible. PMID 6273385.
  3. ↑ Portnoy, D A; Moseley, S L; Falkow, S (1981-02-01). "Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.". Infection and Immunity. 31 (2): 775–782. ISSN 0019-9567. PMC 351377Freely accessible. PMID 7216474.
  4. ↑ Tilney, L. G.; Portnoy, D. A. (1989-10-01). "Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes.". The Journal of Cell Biology. 109 (4): 1597–1608. doi:10.1083/jcb.109.4.1597. ISSN 0021-9525. PMID 2507553.
  5. ↑ Portnoy, D. A.; Jacks, P. S.; Hinrichs, D. J. (1988-04-01). "Role of hemolysin for the intracellular growth of Listeria monocytogenes.". The Journal of Experimental Medicine. 167 (4): 1459–1471. doi:10.1084/jem.167.4.1459. ISSN 0022-1007. PMID 2833557.
  6. ↑ Bielecki, Jacek; Youngman, Philip; Connelly, Patricia; Portnoy, Daniel A. (1990-05-10). "Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells". Nature. 345 (6271): 175–176. Bibcode:1990Natur.345..175B. doi:10.1038/345175a0. PMID 2110628.
  7. ↑ Schafer, R.; Portnoy, D. A.; Brassell, S. A.; Paterson, Y. (1992-07-01). "Induction of a cellular immune response to a foreign antigen by a recombinant Listeria monocytogenes vaccine". Journal of Immunology (Baltimore, Md.: 1950). 149 (1): 53–59. ISSN 0022-1767. PMID 1607662.
  8. ↑ Ikonomidis, G.; Paterson, Y.; Kos, F. J.; Portnoy, D. A. (1994-12-01). "Delivery of a viral antigen to the class I processing and presentation pathway by Listeria monocytogenes". The Journal of Experimental Medicine. 180 (6): 2209–2218. ISSN 0022-1007. PMC 2191788Freely accessible. PMID 7964496.
  9. ↑ "Aduro Biotech | Engineered Immunotherapy for Cancer". aduro.com. Retrieved 2016-09-01.
  10. ↑ "Home - Advaxis". www.advaxis.com. Retrieved 2016-09-01.
  11. ↑ Crimmins, Gregory T.; Herskovits, Anat A.; Rehder, Kai; Sivick, Kelsey E.; Lauer, Peter; Dubensky, Thomas W.; Portnoy, Daniel A. (2008-07-22). "Listeria monocytogenes multidrug resistance transporters activate a cytosolic surveillance pathway of innate immunity". Proceedings of the National Academy of Sciences. 105 (29): 10191–10196. Bibcode:2008PNAS..10510191C. doi:10.1073/pnas.0804170105. ISSN 0027-8424. PMC 2481368Freely accessible. PMID 18632558.
  12. ↑ Woodward, Joshua J.; Iavarone, Anthony T.; Portnoy, Daniel A. (2010-06-25). "c-di-AMP Secreted by Intracellular Listeria monocytogenes Activates a Host Type I Interferon Response". Science. 328 (5986): 1703–1705. Bibcode:2010Sci...328.1703W. doi:10.1126/science.1189801. ISSN 0036-8075. PMC 3156580Freely accessible. PMID 20508090.
  13. ↑ Sauer, John-Demian; Sotelo-Troha, Katia; Moltke, Jakob von; Monroe, Kathryn M.; Rae, Chris S.; Brubaker, Sky W.; Hyodo, Mamoru; Hayakawa, Yoshihiro; Woodward, Joshua J. (2011-02-01). "The N-Ethyl-N-Nitrosourea-Induced Goldenticket Mouse Mutant Reveals an Essential Function of Sting in the In Vivo Interferon Response to Listeria monocytogenes and Cyclic Dinucleotides". Infection and Immunity. 79 (2): 688–694. doi:10.1128/IAI.00999-10. ISSN 0019-9567. PMC 3028833Freely accessible. PMID 21098106.
  14. ↑ "Eli Lilly and Company-Elanco Research Award Past Laureates". www.asm.org. Retrieved 2016-09-01.
  15. ↑ http://www.bostoninteractive.com, Boston Interactive -. "Daniel Portnoy". www.nasonline.org. Retrieved 2016-09-01.

External links

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