Alpha-neoagaro-oligosaccharide hydrolase

Alpha-neoagaro-oligosaccharide hydrolase
Identifiers
EC number 3.2.1.159
CAS number 60063-77-6
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Alpha-neoagaro-oligosaccharide hydrolase (EC 3.2.1.159, alpha-neoagarooligosaccharide hydrolase, alpha-NAOS hydrolase) is an enzyme with systematic name alpha-neoagaro-oligosaccharide 3-glycohydrolase.[1] This enzyme catalyses the following chemical reaction

Hydrolysis of the (1->3)-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose

When neoagarohexaose is used as a substrate, the oligosaccharide is cleaved at the non-reducing end to produce 3,6-anhydro-L-galactose and agaropentaose, which is further hydrolysed to agarobiose and agarotriose.

References

  1. Sugano, Y.; Kodama, H.; Terada, I.; Yamazaki, Y.; Noma, M. (1994). "Purification and characterization of a novel enzyme, α-neoagarooligosaccharide hydrolase (α-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107". J. Bacteriol. 176: 6812–6818. PMID 7961439.

External links

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