2-hydroxymuconate tautomerase

2-hydroxymuconate tautomerase (EC 5.3.2.6, 4-oxalocrotonate tautomerase, 4-oxalocrotonate isomerase, cnbG (gene), praC (gene), xylH (gene)) is an enzyme with systematic name (2Z,4E)-2-hydroxyhexa-2,4-dienedioate keto-enol isomerase.^[1]^[2]^[3]^[4]^[5]^[6] This enzyme catalyses the following chemical reaction

(2Z,4E)-2-hydroxyhexa-2,4-dienedioate

\rightleftharpoons

(3E)-2-oxohex-3-enedioate

Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols.

References

↑ Whitman, C.P.; Aird, B.A.; Gillespie, W.R.; Stolowich, N.J. (1991). "Chemical and enzymatic ketonization of 2-hydroxymuconate, a conjugated enol". J. Am. Chem. Soc. 113: 3154–3162. doi:10.1021/ja00008a052.
↑ Whitman, C.P.; Hajipour, G.; Watson, R.J.; Johnson, W.H.; Jr.; Bembenek, M.E.; Stolowich, N.J. (1992). "Stereospecific ketonization of 2-hydroxymuconate by 4-oxalocrotonate tautomerase and 5-(carboxymethyl)-2-hydroxymuconate isomerase". J. Am. Chem. Soc. 114: 10104–10110. doi:10.1021/ja00052a002.
↑ Subramanya, H.S.; Roper, D.I.; Dauter, Z.; Dodson, E.J.; Davies, G.J.; Wilson, K.S.; Wigley, D.B. (1996). "Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases". Biochemistry. 35 (3): 792–802. doi:10.1021/bi951732k. PMID 8547259.
↑ Stivers, J.T.; Abeygunawardana, C.; Mildvan, A.S.; Hajipour, G.; Whitman, C.P.; Chen, L.H. (1996). "Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies". Biochemistry. 35 (3): 803–813. doi:10.1021/bi951077g. PMID 8547260.
↑ Wang, S.C.; Johnson, W.H.; Jr.; Czerwinski, R.M.; Stamps, S.L.; Whitman, C.P. (2007). "Kinetic and stereochemical analysis of YwhB, a 4-oxalocrotonate tautomerase homologue in Bacillus subtilis: mechanistic implications for the YwhB- and 4-oxalocrotonate tautomerase-catalyzed reactions". Biochemistry. 46 (42): 11919–11929. doi:10.1021/bi701231a. PMID 17902707.
↑ Kasai, D.; Fujinami, T.; Abe, T.; Mase, K.; Katayama, Y.; Fukuda, M.; Masai, E. (2009). "Uncovering the protocatechuate 2,3-cleavage pathway genes". J. Bacteriol. 191: 6758–6768. doi:10.1128/JB.00840-09. PMC 2795304. PMID 19717587.

External links

2-hydroxymuconate tautomerase at the US National Library of Medicine Medical Subject Headings (MeSH)

Isomerases: intramolecular oxidoreductases (EC 5.3)

5.3.1: Aldoses/Ketoses	Triosephosphate isomerase Ribose-5-phosphate isomerase Mannose phosphate isomerase Glucose isomerase

5.3.2: Keto/Enol	Phenylpyruvate tautomerase Oxaloacetate tautomerase

5.3.3: C = C	Steroid D-isomerase Isopentenyl-diphosphate delta isomerase Vinylacetyl-CoA D-isomerase Muconolactone D-isomerase Cholestenol Delta-isomerase (EBP) Methylitaconate D-isomerase Aconitate Delta-isomerase Enoyl CoA isomerase Prostaglandin-A1 Delta-isomerase 5-carboxymethyl-2-hydroxymuconate D-isomerase Isopiperitenone D-isomerase L-dopachrome isomerase Polyenoic fatty acid isomerase

5.3.4: S-S	Protein disulfide-isomerase (PDIA3)

5.3.99: other	Prostaglandin D2 synthase/Prostaglandin-D synthase Prostaglandin E synthase Prostacyclin synthase Thromboxane-A synthase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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