2-haloacid dehalogenase (configuration-inverting)
| 2-haloacid dehalogenase (configuration-inverting) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.8.1.10 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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2-haloacid dehalogenase (configuration-inverting) (EC 3.8.1.10, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, DL-2-haloacid dehalogenase, DL-2-haloacid dehalogenase (inversion of configuration), DL-2-haloacid halidohydrolase (inversion of configuration), DL-DEXi, (R,S)-2-haloacid dehalogenase (configuration-inverting)) is an enzyme with systematic name (S)-2-haloacid dehalogenase (configuration-inverting).[1][2][3][4][5][6][7][8][9] This enzyme catalyses the following chemical reaction
- (1) (S)-2-haloacid + H2O (R)-2-hydroxyacid + halide
- (2) (R)-2-haloacid + H2O (S)-2-hydroxyacid + halide
This enzyme dehalogenates both (S)- and (R)-2-haloalkanoic acids.
References
- ↑ Motosugi, K.; Esahi, N.; Soda, K. (1982). "Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase". Arch. Microbiol. 131: 179–183. doi:10.1007/BF00405875. PMID 7103659.
- ↑ Motosugi, K.; Esahi, N.; Soda, K. (1982). "Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp.". J. Bacteriol. 150 (2): 522–527. PMID 7068529.
- ↑ Motosugi, K.; Esahi, N.; Soda, K. (1984). "Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid". Biotechnol. Bioeng. 26 (7): 805–806. doi:10.1002/bit.260260729.
- ↑ Kurihara, T.; Esaki, N.; Soda, K. (2000). "Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms". J. Mol. Catal., B Enzym. 10: 57–65. doi:10.1016/S1381-1177(00)00108-9.
- ↑ Liu, J.-Q.; Kurihara, T.; Hasan, A.K.M.Q.; Nardi-Dei, V.; Koshikawa, H.; Esaki, N.; Soda, K. (1994). "Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL". Appl. Environ. Microbiol. 60 (7): 2389–2393. PMID 8074519.
- ↑ Cairns, S.S.; Cornish, A.; Cooper, R.A. (1996). "Cloning, sequencing and expression in Escherichia coli of two Rhizobium sp. genes encoding haloalkanoate dehalogenases of opposite stereospecificity". Eur. J. Biochem. 235 (3): 744–749. doi:10.1111/j.1432-1033.1996.t01-1-00744.x. PMID 8654424.
- ↑ Leigh, J.A.; Skinner, A.J.; Cooper, R.A. (1988). "Partial purification, stereospecificity and stoichiometry of three dehalogenases from a Rhizobium species". FEMS Microbiol. Lett. 49: 353–356. doi:10.1111/j.1574-6968.1988.tb02756.x.
- ↑ Weightman, A.J.; Weightman, A.L.; Slater, J.H. (1982). "Stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of Pseudomonas P3: evidence for two different dehalogenation mechanisms". J. Gen. Microbiol. 128 (8): 1755–1762. doi:10.1099/00221287-128-8-1755. PMID 7142958.
- ↑ Soda, K.; Kurihara, T.; Liu, J.-Q.; Nardi-Dei, V.; Park, C.; Miyagi, M.; Tsunasawa, S.; Esaki, N. (1996). "Bacterial 2-haloacid dehalogenases: Structures and catalytic properties". Pure Appl. Chem. 68: 2097–2103. doi:10.1351/pac199668112097.
External links
- 2-haloacid dehalogenase (configuration-inverting) at the US National Library of Medicine Medical Subject Headings (MeSH)
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