(+)-epicubenol synthase

(+)-epicubenol synthase (EC 4.2.3.64, farnesyl pyrophosphate cyclase) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((+)-epicubenol-forming).^[1]^[2]^[3]^[4]^[5] This enzyme catalyses the following chemical reaction

(2E,6E)-farnesyl diphosphate + H₂O

\rightleftharpoons

(+)-epicubenol + diphosphate

This enzyme requires Mg²⁺.

References

↑ Cane, D.E.; Tandon, M.; Prabhakaran, P.C. (1993). "Epicubenol synthase and the enzymatic cyclization of farnesyl diphosphate". J. Am. Chem. Soc. 115: 8103–8106. doi:10.1021/ja00071a023.
↑ Cane, D.E.; Tandon, M. (1994). "Biosynthesis of (+)-epicubenol.". Tetrahedron Lett. 35: 5355–5358. doi:10.1016/s0040-4039(00)73498-8.
↑ Cane, D.E.; Tandon, M. (1995). "Epicubenol synthase and the stereochemistry of the enzymatic cyclization of farnesyl and nerolidyl diphosphate". J. Am. Chem. Soc. 117: 5602–5603. doi:10.1021/ja00125a029.
↑ Nabeta, K.; Kigure, K.; Fujita, M.; Nagoya, T.; Ishikawa, T.; Okuyama, H.; Takasawa, T. (1995). "Bioynthesis of (+)-cubenene and (+)-epicubenol by cell-free extracts of cultured cells of Heteroscyphus planus and cyclization of [²H]farnesyl diphosphates". J. Chem. Soc., Perkin Trans. 1: 1935–1939. doi:10.1039/p19950001935.
↑ Nabeta, K.; Fujita, M.; Komuro, K.; Katayama, K.; Takasawa, T. (1997). "In vitro biosynthesis of cadinanes by cell-free extracts of cultured cells of Heteroscyphus planus". J. Chem. Soc., Perkin Trans. 1: 2065–2070. doi:10.1039/a701145h.

Carbon-oxygen lyases (EC 4.2) (primarily dehydratases)

4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase

4.2.2: Acting on polysaccharides	Hyaluronate lyase

4.2.3: Acting on phosphates	Threonine synthase

4.2.99: Other	Carboxymethyloxysuccinate lyase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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